3DPU
RocCOR domain tandem of Rab family protein (Roco)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97892 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.200, 106.100, 139.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.670 - 2.900 |
| R-factor | 0.23226 |
| Rwork | 0.230 |
| R-free | 0.27005 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dpt |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.071 | 0.466 |
| Number of reflections | 27475 | |
| <I/σ(I)> | 20.52 | 4.2 |
| Completeness [%] | 99.0 | 99.7 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M HEPES (pH7.0), 1M Na citrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
