3DPS

X-ray structure of the unliganded uridine phosphorylase from salmonella typhimurium in homodimeric form at 1.8A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-23
Detector	MAR CCD 165 mm
Wavelength(s)	0.813
Spacegroup name	H 3
Unit cell lengths	150.860, 150.860, 47.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.696 - 1.800
R-factor	0.163
Rwork	0.161
R-free	0.20000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oxf
RMSD bond length	0.006
RMSD bond angle	1.042
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.800
Rmerge	0.058
Number of reflections	37497
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	295	PEG 400, NaN3, GLYCEROL, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K