3DPG
SgrAI with noncognate DNA bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Wavelength(s) | 1.08 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.726, 78.621, 86.834 |
Unit cell angles | 90.00, 109.76, 90.00 |
Refinement procedure
Resolution | 31.257 - 1.910 |
R-factor | 0.1867 |
Rwork | 0.185 |
R-free | 0.22540 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.958 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | DM |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.910 |
Rmerge | 0.071 |
Number of reflections | 66598 |
<I/σ(I)> | 6.8 |
Completeness [%] | 96.1 |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | PEG4000, NaCl, CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG4000 | ||
2 | 1 | 1 | NaCl | ||
3 | 1 | 1 | CaCl2 | ||
4 | 1 | 2 | PEG4000 | ||
5 | 1 | 2 | NaCl | ||
6 | 1 | 2 | CaCl2 |