3DP4
Crystal structure of the binding domain of the AMPA subunit GluR3 bound to AMPA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-08 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 47.342, 47.342, 138.268 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.110 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.26000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1FTJ A PROTOMER |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.180 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.082 | 0.331 |
| Number of reflections | 18484 | |
| <I/σ(I)> | 24.1 | 6.73 |
| Completeness [%] | 96.0 | 100 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 277 | 15-17% PEG 1450, 0.2 M Zn acetate, 0.2 M Ammonium sulfate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
