3DOU
Crystal structure of methyltransferase involved in cell division from thermoplasma volcanicum gss1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2007-06-27 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.620, 50.053, 56.457 |
| Unit cell angles | 90.00, 108.08, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.450 |
| R-factor | 0.1671 |
| Rwork | 0.166 |
| R-free | 0.19456 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.484 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.062 | 0.726 |
| Number of reflections | 30605 | |
| <I/σ(I)> | 5.4 | 1.2 |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 100MM HEPES,PH 7.5, 20% PEG3350, 10% GLYCEROL, VAPOR DIFFUSION, TEMPERATURE 298K, pH 7.50 |
