3DOM

Crystal Structure of the complex between Tfb5 and the C-terminal domain of Tfb2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97923
Spacegroup name	P 21 21 21
Unit cell lengths	37.583, 103.593, 114.345
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.700 - 2.600
R-factor	0.205
Rwork	0.202
R-free	0.26100
RMSD bond length	0.016
RMSD bond angle	1.826
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	RESOLVE
Refinement software	REFMAC (5.4.0067)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.058	0.041	0.131
Number of reflections	13927
<I/σ(I)>	23.7
Completeness [%]	96.4	89.7	91.9
Redundancy	4	3.9	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	PEG3350 MME, NaCl, Hepes, pH 7.5, vapor diffusion, sitting drop, temperature 293K