3DOJ
Structure of Glyoxylate reductase 1 from Arabidopsis (AtGLYR1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97934 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 156.543, 156.543, 75.467 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.610 - 2.101 |
R-factor | 0.172 |
Rwork | 0.172 |
R-free | 0.18800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2uyy |
RMSD bond length | 0.004 |
RMSD bond angle | 0.817 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.065 | 0.410 |
Number of reflections | 27496 | |
<I/σ(I)> | 11.5 | 4.1 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 8.4 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M calcium acetate hydrate, 20% PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |