3DOE
Complex of ARL2 and BART, Crystal Form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-12-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.5596 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.827, 44.081, 66.495 |
| Unit cell angles | 90.00, 111.46, 90.00 |
Refinement procedure
| Resolution | 35.900 - 2.250 |
| R-factor | 0.23168 |
| Rwork | 0.230 |
| R-free | 0.26123 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ksg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.327 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.065 | 0.216 |
| Number of reflections | 14727 | |
| <I/σ(I)> | 27.9 | 4 |
| Completeness [%] | 97.7 | 83.7 |
| Redundancy | 6.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 0.1M bicine, 10% PEG 6000, 20mM hexammine cobalt (III) chloride, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
