3DOD
Crystal Structure of PLP Bound 7,8-Diaminopelargonic Acid Synthase in Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.674, 104.027, 73.868 |
| Unit cell angles | 90.00, 105.38, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.900 |
| R-factor | 0.19794 |
| Rwork | 0.196 |
| R-free | 0.24022 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bv0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.268 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.800 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.072 | 0.480 |
| Number of reflections | 65688 | |
| <I/σ(I)> | 10.5 | 2.8 |
| Completeness [%] | 99.1 | 98 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 20% PEG3350, 0.2M sodium thiocyanate, 5% xylitol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
