3DO5

Crystal structure of Putative Homoserine Dehydrogenase (NP_069768.1) from ARCHAEOGLOBUS FULGIDUS at 2.20 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-14
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	C 2 2 21
Unit cell lengths	119.107, 120.818, 57.886
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.940 - 2.200
R-factor	0.189
Rwork	0.188
R-free	0.22100
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.810
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.940	28.960	2.260
High resolution limit [Å]	2.200	9.840	2.200
Rmerge	0.087	0.053	0.520
Total number of observations		1609	7519
Number of reflections	21542
<I/σ(I)>	5.4	11.6	1.3
Completeness [%]	99.7	94.3	99.5
Redundancy	7.4	6.1	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	0.2M ammonium sulfate, 10.0% Glycerol, 20.0% polyethylene glycol 300, 0.1M phosphate-citrate pH 4.2, NANODROP', VAPOR DIFFUSION, SITTING DROP, temperature 293K