3DO3
Human 1gG1 Fc fragment, 2.5 Angstrom structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-11-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.205, 80.216, 138.749 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.500 - 2.500 |
| R-factor | 0.28 |
| Rwork | 0.280 |
| R-free | 0.33000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hwz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.314 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.500 | 2.580 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.028 | 0.170 |
| Number of reflections | 19709 | |
| Completeness [%] | 98.0 | 93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 298 | 20% PEG 3350, 0.2M NaCl pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
