3DO0
Thermolysin by classical hanging drop method after high X-Ray dose on esrf ID14-2 beamline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-05-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 92.724, 92.724, 128.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 80.320 - 1.360 |
| R-factor | 0.224 |
| Rwork | 0.223 |
| R-free | 0.25500 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.202 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.322 | 1.380 |
| High resolution limit [Å] | 1.360 | 1.310 |
| Rmerge | 0.275 | 0.154 |
| Total number of observations | 3967 | |
| Number of reflections | 64386 | |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 81.2 | 24.1 |
| Redundancy | 5.3 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | DMSO, Ammonium sulfate, MES, hanging drop, 293K, pH6.0, VAPOR DIFFUSION, HANGING DROP |
