3DNX
SPO1766 protein of unknown function from Silicibacter pomeroyi.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 59.892, 59.892, 99.868 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.000 - 1.940 |
R-factor | 0.174 |
Rwork | 0.172 |
R-free | 0.21300 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.523 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.000 | 1.970 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.106 | 0.820 |
Number of reflections | 14100 | |
<I/σ(I)> | 10.2 | 3.01 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.8 | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 277 | 2.5 M sodium chloride, 0.1 M sodium/potassium phosphate buffer, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |