3DNU
structure of MDT protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9797, 1.02, 0.9796 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.660, 85.810, 49.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.990 - 1.540 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.23200 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 1.640 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.054 | 0.380 |
| Number of reflections | 59003 | |
| <I/σ(I)> | 13.7 | 2 |
| Completeness [%] | 98.0 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | peg 8000, glycerol, phosphate buffer, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
