3DNK
Enzyme deglycosylated Human IgG1 Fc fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.370, 78.210, 147.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.080 - 2.840 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.32000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.940 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmerge | 0.049 | 0.163 |
| Number of reflections | 13869 | |
| Completeness [%] | 98.0 | 82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 298 | 20% PEG 6000, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
