3DNJ
The structure of the Caulobacter crescentus ClpS protease adaptor protein in complex with a N-end rule peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97927 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.693, 53.974, 44.824 |
Unit cell angles | 90.00, 110.39, 90.00 |
Refinement procedure
Resolution | 14.876 - 1.150 |
R-factor | 0.136 |
Rwork | 0.135 |
R-free | 0.15900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1R60 chain C |
RMSD bond length | 0.006 |
RMSD bond angle | 0.876 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.190 |
High resolution limit [Å] | 1.150 | 2.480 | 1.150 |
Rmerge | 0.078 | 0.061 | 0.194 |
Number of reflections | 51120 | ||
<I/σ(I)> | 8.1 | ||
Completeness [%] | 95.6 | 95.2 | 69.5 |
Redundancy | 6.3 | 6.4 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.1 M BIS-TRIS PH 5.5 0.2 M MGCL2 13% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K |