3DNI

CRYSTALLOGRAPHIC REFINEMENT AND STRUCTURE OF DNASE I AT 2 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	131.600, 54.900, 38.400
Unit cell angles	90.00, 91.40, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.177
RMSD bond length	0.023
RMSD bond angle	0.030
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	2.500 *
High resolution limit [Å]	2.000 *
Rmerge	0.064 *
Number of reflections	9025 *
Completeness [%]	86.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.6 *	19 *	referred to J.Mol.Biol. 162.511-513 1982 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	2	PEG6000	11 (%)
2	1	2	imidazole	70 (mM)
3	1	2		100 (mM)
4	1	2		0.1 (mM)
5	1	2		0.1 (mM)
6	1	1	protein	3.5 (mg/ml)
7	1	2	enzyme