3DN7
Cyclic nucleotide binding regulatory protein from Cytophaga hutchinsonii.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.728, 83.519, 89.633 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.800 - 1.800 |
| R-factor | 0.173 |
| Rwork | 0.172 |
| R-free | 0.19000 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.607 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.900 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.081 | 0.686 |
| Number of reflections | 30873 | |
| <I/σ(I)> | 10.3 | 2.02 |
| Completeness [%] | 98.9 | 87.6 |
| Redundancy | 6.8 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.1 mM HEPES buffer, 10% PEG 6000, 5 mM cAMP, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
