3DMU
Crystal structure of Staphylococcal nuclease variant PHS T62K at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-01-20 |
Detector | BRUKER APEX II |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 |
Unit cell lengths | 48.220, 48.220, 63.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.000 - 1.800 |
R-factor | 0.203 |
Rwork | 0.198 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1EY8.PDB |
RMSD bond length | 0.009 |
RMSD bond angle | 2.080 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0017) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.980 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.030 | 0.284 |
Number of reflections | 14710 | |
<I/σ(I)> | 30.16 | 4.05 |
Completeness [%] | 99.8 | 99.3 |
Redundancy | 10.6 | 3.43 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 44% MPD, 25 mM Potassium Phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |