3DMC
CRYSTAL STRUCTURE OF A NTF2-LIKE PROTEIN (AVA_2261) FROM ANABAENA VARIABILIS ATCC 29413 AT 1.65 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-27 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.777, 42.138, 46.342 |
| Unit cell angles | 87.97, 65.83, 83.74 |
Refinement procedure
| Resolution | 29.907 - 1.650 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.22400 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.462 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.907 | 29.910 | 1.690 |
| High resolution limit [Å] | 1.650 | 7.380 | 1.650 |
| Rmerge | 0.075 | 0.053 | 0.412 |
| Total number of observations | 1235 | 5830 | |
| Number of reflections | 28896 | ||
| <I/σ(I)> | 7 | 10.5 | 1.9 |
| Completeness [%] | 92.7 | 97.6 | 65.8 |
| Redundancy | 3.9 | 3.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.69 | 293 | 0.17M di-ammonium hydrogen phosphate, 0.1M sodium acetate pH 4.69, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
