3DM8
Crystal Structure of Putative Isomerase from Rhodopseudomonas palustris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-08 |
| Detector | APS-1 |
| Wavelength(s) | 0.97912 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 40.593, 84.071, 97.211 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.620 - 1.800 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.21600 |
| Structure solution method | SIR/SIRAS |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.705 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.620 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.057 | 0.322 |
| Number of reflections | 15870 | |
| <I/σ(I)> | 35 | 6.2 |
| Completeness [%] | 99.9 | 96.3 |
| Redundancy | 7.5 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 294 | PEG400 15%, MgCl2 0.2M, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, temperature 294K |
