3DLL
The oxazolidinone antibiotics perturb the ribosomal peptidyl-transferase center and effect tRNA positioning
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 169.700, 410.000, 695.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.980 - 3.500 |
R-factor | 0.259 |
Rwork | 0.259 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zjr |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.770 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 272947 | |
<I/σ(I)> | 8.2 | 1.9 |
Completeness [%] | 90.5 | 80.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 10mM MgCl2, 60mM NH4Cl, 5mM KCl, 10mM HEPES, pH7.8, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MgCl2 | ||
2 | 1 | 1 | NH4Cl | ||
3 | 1 | 1 | KCl | ||
4 | 1 | 1 | HEPES | ||
5 | 1 | 2 | MgCl2 | ||
6 | 1 | 2 | NH4Cl | ||
7 | 1 | 2 | KCl | ||
8 | 1 | 2 | HEPES |