3DLI
Crystal structure of a SAM dependent methyltransferase from Archaeoglobus fulgidus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.154, 81.880, 122.797 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.120 - 2.460 |
R-factor | 0.23 |
Rwork | 0.230 |
R-free | 0.28600 |
Structure solution method | SAD |
Starting model (for MR) | None |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.550 |
High resolution limit [Å] | 2.460 | 2.460 |
Rmerge | 0.080 | 0.500 |
Number of reflections | 28155 | |
<I/σ(I)> | 9.9 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.3 | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2M Ammonium sulfate, 0.1M Bis-tris, 25% PEG 3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |