3DLH
Crystal structure of the guide-strand-containing Argonaute protein silencing complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97926 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.744, 114.629, 175.689 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.000 |
Rwork | 0.226 |
R-free | 0.28400 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.634 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.089 | 0.447 |
Number of reflections | 30932 | |
<I/σ(I)> | 14 | 2 |
Completeness [%] | 99.3 | 95.8 |
Redundancy | 4.5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 308 | 18% PEG8000, 200mM KCl, 80mM MgAc2, 50mM Mes, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 308K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Mes | ||
2 | 1 | 2 | Mes | ||
3 | 1 | 2 | KCl | ||
4 | 1 | 2 | MgAc2 | ||
5 | 1 | 2 | PEG8000 |