3DLC
Crystal structure of a putative s-adenosyl-l-methionine-dependent methyltransferase (mmp1179) from methanococcus maripaludis at 1.15 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-15 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837,0.97947 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.610, 63.500, 75.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.260 - 1.150 |
R-factor | 0.121 |
Rwork | 0.120 |
R-free | 0.13800 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.723 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.260 | 1.190 | |
High resolution limit [Å] | 1.150 | 2.480 | 1.150 |
Rmerge | 0.037 | 0.025 | 0.378 |
Number of reflections | 68022 | 12763 | 11988 |
<I/σ(I)> | 11.43 | 39.8 | 2.3 |
Completeness [%] | 98.0 | 97.8 | 94.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.17M ammonium acetate, 15.0% Glycerol, 25.5% polyethylene glycol 4000, 0.1M sodium acetate pH 4.6, Additive - 1mM S-adenosylmethionine, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |