3DLB

Crystal structure of the guide-strand-containing Argonaute protein silencing complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-27
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97918, 0.97929
Spacegroup name	P 1 21 1
Unit cell lengths	77.846, 123.399, 84.910
Unit cell angles	90.00, 95.70, 90.00

Refinement procedure

Resolution	30.000 - 2.700
Rwork	0.254
R-free	0.29310
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.874
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge		0.643
Number of reflections	42559
<I/σ(I)>	29.4	1.72
Completeness [%]		100
Redundancy	4.5	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	308	5% PEGMME550, 0.1M KCl, 10mM MgCl2, 50 mM Tris HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 308K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MgCl2
2	1	1	Tris HCl
3	1	2	MgCl2
4	1	2	Tris HCl
5	1	2	PEGMME550
6	1	2	KCl