3DLB
Crystal structure of the guide-strand-containing Argonaute protein silencing complex
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-27 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97918, 0.97929 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.846, 123.399, 84.910 |
Unit cell angles | 90.00, 95.70, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.700 |
Rwork | 0.254 |
R-free | 0.29310 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.874 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.643 | |
Number of reflections | 42559 | |
<I/σ(I)> | 29.4 | 1.72 |
Completeness [%] | 100 | |
Redundancy | 4.5 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 308 | 5% PEGMME550, 0.1M KCl, 10mM MgCl2, 50 mM Tris HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 308K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MgCl2 | ||
2 | 1 | 1 | Tris HCl | ||
3 | 1 | 2 | MgCl2 | ||
4 | 1 | 2 | Tris HCl | ||
5 | 1 | 2 | PEGMME550 | ||
6 | 1 | 2 | KCl |