3DL3
Crystal structure of the tellurite resistance protein TehB. Northeast Structural Genomics Consortium target VfR98 .
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.574, 205.020, 56.356 |
| Unit cell angles | 90.00, 110.21, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.300 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.27400 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.270 |
| Rmerge | 0.072 | |
| Number of reflections | 82534 | |
| <I/σ(I)> | 19.6 | 3.1 |
| Completeness [%] | 97.3 | |
| Redundancy | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 293 | 0.1M MgCl2 0.1M MES 40% PEG400, pH 6.0, Crystals were grown by microbatch under oil method. temperature 293K | |
| 1 | 6 | 293 | 0.1M MgCl2 0.1M MES 40% PEG400, pH 6.0, Crystals were grown by microbatch under oil method. temperature 293K |
