3DL2
Hexagonal structure of the LDH domain of Human Ubiquitin-conjugating Enzyme E2-like Isoform A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-11 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 65 |
| Unit cell lengths | 105.603, 105.603, 131.918 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.970 - 2.100 |
| R-factor | 0.1962 |
| Rwork | 0.195 |
| R-free | 0.22550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i10 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.006 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 47978 | |
| <I/σ(I)> | 28.929 | 3.32 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.2 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 290.9 | 1.6 M Sodium/potassium phosphate pH 6.5, 0.2 M Sodium chloride, 0.002 M Dithiothreitol, VAPOR DIFFUSION, HANGING DROP, temperature 290.9K |
