3DKY
Crystal Structure of the replication initiator protein encoded on plasmid pMV158 (RepB), tetragonal form, to 3.6 Ang resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.005 |
| Spacegroup name | P 43 |
| Unit cell lengths | 91.460, 91.460, 227.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.571 - 3.600 |
| R-factor | 0.2251 |
| Rwork | 0.223 |
| R-free | 0.25670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dkx |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.670 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.850 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.075 | 0.462 |
| Total number of observations | 7763 | |
| Number of reflections | 21350 | |
| <I/σ(I)> | 10.95 | 3.01 |
| Completeness [%] | 97.8 | 98.9 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | 10% PEG 4000, 0.2mM MgCl2, 50mM CHES, pH 9, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
