3DKX

Crystal Structure of the replication initiator protein encoded on plasmid pMV158 (RepB), trigonal form, to 2.7 Ang resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-23
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0052
Spacegroup name	P 32 2 1
Unit cell lengths	85.611, 85.611, 245.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.950 - 2.700
R-factor	0.23048
Rwork	0.228
R-free	0.27574
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.478
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.950	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.100	0.748
Number of reflections	29572
<I/σ(I)>	15.1	2.4
Completeness [%]	99.9	100
Redundancy	6.5	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	10% PEG 8000, 100 mM CaCl2, 50mM TRIS HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	10% PEG 8000, 100 mM CaCl2, 50mM TRIS HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	10% PEG 8000, 100 mM CaCl2, 50mM TRIS HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	10% PEG 8000, 100 mM CaCl2, 50mM TRIS HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	10% PEG 8000, 100 mM CaCl2, 50mM TRIS HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K