3DKS
DsbA substrate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-11-09 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.433, 87.938, 112.566 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.340 - 1.900 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dsb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.020 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.340 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 67234 | |
| <I/σ(I)> | 15.8 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.1 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277.15 | 0.2M AMMONIUM ACETATE, 20%(W/V) PEG 4000, 0.1M sodium citrate(pH 5.8), pH 6.700000, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
