3DKP
Human DEAD-box RNA-helicase DDX52, conserved domain I in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.630, 38.360, 73.840 |
| Unit cell angles | 90.00, 90.37, 90.00 |
Refinement procedure
| Resolution | 19.560 - 2.100 |
| R-factor | 0.18451 |
| Rwork | 0.182 |
| R-free | 0.23636 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2db3 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.414 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.088 | 0.378 |
| Number of reflections | 13480 | |
| <I/σ(I)> | 12.8 | 4.61 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 3.65 | 3.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 10% PEG 8000, 100 mM Imidazole, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
