3DK5
Crystal Structure of Apo-GlmU from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-17 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | H 3 |
| Unit cell lengths | 79.600, 79.600, 278.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.300 - 2.230 |
| R-factor | 0.227 |
| Rwork | 0.225 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.260 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.290 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.123 | 0.723 |
| Number of reflections | 31336 | 1920 |
| <I/σ(I)> | 11.3 | 2.4 |
| Completeness [%] | 98.2 | 80.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 8% PEG 8000, 150mM NaCl, 2mM MnCl2, 5%Glycerol, 1,3-butanediol, AMPPNP, MgCl2, DTT, pH 7.5, vapor diffusion, sitting drop, temperature 277K |
