3DK0
Crystal structure of transthyretin variant L55P at acidic pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9340 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 42.632, 84.780, 65.415 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.370 - 1.870 |
| R-factor | 0.20768 |
| Rwork | 0.207 |
| R-free | 0.22250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f41 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.001 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.300 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.062 | 0.434 |
| Number of reflections | 19735 | |
| <I/σ(I)> | 19.8 | 3.5 |
| Completeness [%] | 98.1 | 89.7 |
| Redundancy | 6.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 295 | 32% PEG 400, 0.05-0.2M CaCl2, 0.1M NaHepes , pH 4.6, VAPOR DIFFUSION, temperature 295K |
