3DJX
Bovine Seminal Ribonuclease- cytidine 5' phosphate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8088 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.585, 59.016, 82.247 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.770 - 1.690 |
| R-factor | 0.17036 |
| Rwork | 0.169 |
| R-free | 0.20428 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1r5d |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.284 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.775 | 1.730 |
| High resolution limit [Å] | 1.690 | 1.700 |
| Number of reflections | 26554 | |
| <I/σ(I)> | 14.1 | 3.4 |
| Completeness [%] | 98.1 | 98.7 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 30% PEG4000, 0.1M sodium acetate, 0.1M Tris/HCl, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
