3DJM
CRYSTAL STRUCTURE OF A PROTEIN OF UNKNOWN FUNCTION FROM DUF427 FAMILY (RSPH17029_0682) FROM RHODOBACTER SPHAEROIDES 2.4.1 AT 2.51 A RESOLUTION
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-16 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91162,0.97929,0.97915 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.403, 93.087, 128.016 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.831 - 2.510 |
R-factor | 0.213 |
Rwork | 0.212 |
R-free | 0.22800 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.492 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.831 | 29.480 | 2.580 |
High resolution limit [Å] | 2.510 | 11.230 | 2.510 |
Rmerge | 0.073 | 0.029 | 0.681 |
Total number of observations | 1106 | 7168 | |
Number of reflections | 27752 | ||
<I/σ(I)> | 8.3 | 20.3 | 1.1 |
Completeness [%] | 99.6 | 92.6 | 99.2 |
Redundancy | 3.6 | 3.2 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20.0% polyethylene glycol 3350, 0.2M sodium formate, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |