3DIY
Crystallization of the Thermotoga maritima lysine riboswitch bound to lysine, Mn2+ soak
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-17 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.24 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.193, 78.662, 140.522 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.710 |
R-factor | 0.19099 |
Rwork | 0.189 |
R-free | 0.23671 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3dil |
RMSD bond length | 0.006 |
RMSD bond angle | 1.239 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.095 | 0.588 |
Number of reflections | 16392 | |
<I/σ(I)> | 21.8 | 2.8 |
Completeness [%] | 96.6 | 95.9 |
Redundancy | 5.9 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | 18% PEG 4000, 0.1M Sodium Citrate, 20% isopropanol, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
2 | 1 | 1 | Sodium Citrate | ||
3 | 1 | 1 | isopropanol | ||
4 | 1 | 2 | PEG 4000 | ||
5 | 1 | 2 | Sodium Citrate | ||
6 | 1 | 2 | isopropanol |