3DIY
Crystallization of the Thermotoga maritima lysine riboswitch bound to lysine, Mn2+ soak
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.24 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.193, 78.662, 140.522 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.710 |
| R-factor | 0.19099 |
| Rwork | 0.189 |
| R-free | 0.23671 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3dil |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.239 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.095 | 0.588 |
| Number of reflections | 16392 | |
| <I/σ(I)> | 21.8 | 2.8 |
| Completeness [%] | 96.6 | 95.9 |
| Redundancy | 5.9 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | 18% PEG 4000, 0.1M Sodium Citrate, 20% isopropanol, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | Sodium Citrate | ||
| 3 | 1 | 1 | isopropanol | ||
| 4 | 1 | 2 | PEG 4000 | ||
| 5 | 1 | 2 | Sodium Citrate | ||
| 6 | 1 | 2 | isopropanol |
