3DIP
Crystal structure of an enolase protein from the environmental genome shotgun sequencing of the Sargasso Sea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97958 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 282.292, 282.292, 282.292 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.218 |
| Rwork | 0.215 |
| R-free | 0.27500 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.367 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.727 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.103 | 0.295 |
| Total number of observations | 208049 | |
| Number of reflections | 33847 | |
| <I/σ(I)> | 30.5 | 12.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 42.2 | 43.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 294 | 100mM Tris HCl pH 8.0, 1.5M ammonium sulfate, Vapor diffusion, temperature 294K |
