3DID
Crystal structure of the F87M/L110M mutant of human transthyretin at pH 4.6 soaked
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.425 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.030, 63.470, 48.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.070 - 1.780 |
| R-factor | 0.185 |
| Rwork | 0.181 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.035 |
| RMSD bond angle | 2.165 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.066 | 1.880 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.052 | 0.506 |
| Total number of observations | 21553 | |
| Number of reflections | 48266 | |
| <I/σ(I)> | 6.6 | 1.5 |
| Completeness [%] | 96.1 | 93.7 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | ZINC ACETATE 0.2 M, SODIUM CITRATE 0.1 M, AMMONIUM SULFATE 2,0M, PH 4,6; CRYSTALS WERE SOAKED IN MOTHER LIQUOR SUPPLEMENTED WITH 10 % GLYCEROL BEFORE FREEZING IN LIQUID NITROGEN, pH 4.60, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
