3DHR
Crystal Structure Determination of Methemoglobin from Pigeon at 2 Angstrom Resolution (Columba livia)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-05-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.659, 65.322, 103.733 |
| Unit cell angles | 78.78, 89.90, 65.30 |
Refinement procedure
| Resolution | 19.990 - 2.000 |
| R-factor | 0.24663 |
| Rwork | 0.243 |
| R-free | 0.27597 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1faw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.933 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 79185 | |
| <I/σ(I)> | 6.83 | |
| Redundancy | 1.75 | 1.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 30% PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
