3DH4

Crystal Structure of Sodium/Sugar symporter with bound Galactose from vibrio parahaemolyticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97930
Spacegroup name	P 1
Unit cell lengths	83.280, 109.210, 127.580
Unit cell angles	109.70, 92.02, 102.11

Refinement procedure

Resolution	30.000 - 2.700
R-factor	0.27063
Rwork	0.270
R-free	0.28715
Structure solution method	SIRAS
RMSD bond length	0.009
RMSD bond angle	1.071
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.113	0.429
Number of reflections	103009
<I/σ(I)>	8.6	1.55
Completeness [%]	91.3	69.9
Redundancy	4	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	278	100 mM Hepes, 200mM Ca Acetate, 20-25% PEG 400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 278K
1	VAPOR DIFFUSION, HANGING DROP	7	278	100 mM Hepes, 200mM Ca Acetate, 20-25% PEG 400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 278K