3DGP
Crystal Structure of the complex between Tfb5 and the C-terminal domain of Tfb2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9198 |
| Spacegroup name | P 61 |
| Unit cell lengths | 99.992, 99.992, 37.262 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.19905 |
| Rwork | 0.197 |
| R-free | 0.23708 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.270 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.870 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.249 |
| Number of reflections | 19737 | |
| <I/σ(I)> | 26 | 7.3 |
| Completeness [%] | 98.5 | 0.937 |
| Redundancy | 10.3 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.9 | 277 | Tris 20 mM, NaCl 50 mM, pH 8.9, EVAPORATION, temperature 277K |
