3DGF
Structure of a histidine kinase-response regulator complex reveals insights into Two-component signaling and a novel cis-autophosphorylation mechanism
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM16 |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2005-01-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.008 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.574, 55.476, 58.428 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.100 - 2.000 |
R-factor | 0.214 |
Rwork | 0.211 |
R-free | 0.25900 |
Starting model (for MR) | RR468 from the entry 2008-Jun-12.09.34.07.979392.Session.11388 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.600 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.100 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.088 | 0.383 |
Number of reflections | 40193 | |
<I/σ(I)> | 8.8 | 3.9 |
Completeness [%] | 99.3 | 100 |
Redundancy | 5.1 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 28% PEG8000, 0.2M ammonium sulfate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |