3DG1
Segment SSTNVG derived from IAPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 41.400, 4.785, 18.594 |
| Unit cell angles | 90.00, 115.88, 90.00 |
Refinement procedure
| Resolution | 18.620 - 1.660 |
| R-factor | 0.211 |
| Rwork | 0.207 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | poly-alanine beta strand |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.011 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.170 | 0.524 |
| Number of reflections | 460 | |
| <I/σ(I)> | 5.5 | |
| Completeness [%] | 97.9 | 97.4 |
| Redundancy | 2.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% MPD, no buffer, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
