3DFV

Adjacent GATA DNA binding

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Detector technology	CCD
Collection date	2006-03-12
Detector	ADSC QUANTUM 210
Wavelength(s)	1.07810
Spacegroup name	C 1 2 1
Unit cell lengths	137.977, 35.756, 54.487
Unit cell angles	90.00, 113.25, 90.00

Refinement procedure

Resolution	30.000 - 3.100
Rwork	0.276
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.210
High resolution limit [Å]	3.100	6.660	3.100
Rmerge	0.078	0.044	0.164
Number of reflections	4593
<I/σ(I)>	13
Completeness [%]	99.9	99.2	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	290	20 mM Mg(OAc), Cacodylic acid, 30% PEG 4K, pH 6.5, hanging drop, temperature 290K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	Cacodylic acid
2	1	1	Mg(OAc)
3	1	2	Cacodylic acid
4	1	2	Mg(OAc)
5	1	2	PEG 4K