3DFV
Adjacent GATA DNA binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Detector technology | CCD |
| Collection date | 2006-03-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.07810 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.977, 35.756, 54.487 |
| Unit cell angles | 90.00, 113.25, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.100 |
| Rwork | 0.276 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 6.660 | 3.100 |
| Rmerge | 0.078 | 0.044 | 0.164 |
| Number of reflections | 4593 | ||
| <I/σ(I)> | 13 | ||
| Completeness [%] | 99.9 | 99.2 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 290 | 20 mM Mg(OAc), Cacodylic acid, 30% PEG 4K, pH 6.5, hanging drop, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Cacodylic acid | ||
| 2 | 1 | 1 | Mg(OAc) | ||
| 3 | 1 | 2 | Cacodylic acid | ||
| 4 | 1 | 2 | Mg(OAc) | ||
| 5 | 1 | 2 | PEG 4K |
