3DFQ
D33S mutant fructose-1,6-bisphosphate aldolase from rabbit muscle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-15 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.854, 103.719, 84.822 |
Unit cell angles | 90.00, 98.81, 90.00 |
Refinement procedure
Resolution | 35.290 - 1.820 |
Rwork | 0.150 |
R-free | 0.18800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zah |
RMSD bond length | 0.004 |
RMSD bond angle | 1.208 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.750 |
High resolution limit [Å] | 1.690 | 1.690 |
Number of reflections | 143542 | |
<I/σ(I)> | 18 | 1.8 |
Completeness [%] | 89.6 | 50.1 |
Redundancy | 3 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | sodium HEPES, PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |