3DFG
Crystal Structure of RecX: A Potent Inhibitor Protein of RecA from Xanthomonas campestris
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Wavelength(s) | 0.97997, 0.96472 |
Spacegroup name | P 43 |
Unit cell lengths | 45.100, 45.100, 93.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.350 - 1.500 |
Rwork | 0.198 |
R-free | 0.22730 |
RMSD bond length | 0.004 |
RMSD bond angle | 1.000 |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.056 | |
Number of reflections | 55383 | |
<I/σ(I)> | 4 | |
Completeness [%] | 99.4 | |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |