3DEP
Structural basis for specific substrate recognition by the chloroplast signal recognition particle protein cpSRP43
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9310 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 66.473, 66.473, 219.703 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.000 - 2.700 |
R-factor | 0.252 |
Rwork | 0.249 |
R-free | 0.29800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 3DEO |
RMSD bond length | 0.012 |
RMSD bond angle | 1.452 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.103 | 0.451 |
Total number of observations | 8065 | |
Number of reflections | 8435 | |
<I/σ(I)> | 3.6 | 3.6 |
Completeness [%] | 99.1 | 99.6 |
Redundancy | 6.5 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | VAPOR DIFFUSION, HANGING DROP, temperature 293K |