3DDK
Coxsackievirus B3 3Dpol RNA Dependent RNA Polymerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2006-01-13 |
Detector | NOIR-1 |
Wavelength(s) | 1.00 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 74.360, 74.360, 288.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.580 - 2.250 |
R-factor | 0.204 |
Rwork | 0.201 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1RA6 - poliovirus polymerase |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 52.580 | 52.580 | 2.330 |
High resolution limit [Å] | 2.250 | 4.850 | 2.250 |
Rmerge | 0.051 | 0.035 | 0.243 |
Total number of observations | 56328 | 3435 | |
Number of reflections | 35017 | ||
<I/σ(I)> | 33 | 54.6 | 3 |
Completeness [%] | 88.5 | 99.9 | 41.4 |
Redundancy | 9.32 | 12.64 | 1.78 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 1.5M ammonium sulfate, 25% glycerol, 200 mM NaCl (unbuffered), pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |