3DDJ
Crystal structure of a cbs domain-containing protein in complex with amp (sso3205) from sulfolobus solfataricus at 1.80 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-27 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.560, 48.090, 83.280 |
Unit cell angles | 90.00, 93.83, 90.00 |
Refinement procedure
Resolution | 29.617 - 1.800 |
R-factor | 0.17 |
Rwork | 0.168 |
R-free | 0.20600 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.509 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.617 | 1.860 | |
High resolution limit [Å] | 1.800 | 3.870 | 1.800 |
Rmerge | 0.047 | 0.023 | 0.529 |
Number of reflections | 28983 | 5708 | 4948 |
<I/σ(I)> | 17.67 | 45.3 | 2.5 |
Completeness [%] | 99.1 | 99.7 | 93.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.76 | 293 | 23.0% polyethylene glycol 600, 0.1M phosphate-citrate pH 3.76, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |